摘要
目的 分析对于原发性胆汁性胆管炎患者，采用不同的免疫检测方法，其特异性自身抗体的具体性能。方法 选取2018.1月~2020.1月期间，因原发性胆汁性胆管炎疾病住院接受治疗的患者，共计76例，将其作为本次实验的主要研究对象，对于使用不同的免疫方法进行特异性自身抗体的检测。根据随机分组，76例原发性胆汁性胆管炎患者作为观察组，50例肝脏疾病患者和40例健康患者作为对照组，肝脏疾病患者主要是包括了病毒性肝炎、自身免疫性肝炎和肝硬化。使用化学发光法(CLIA)和酶联免疫吸附法(ELISA)对所有患者进行平行检测AMA—M2，并使用化学发光法(CLIA)和线性免疫印迹法(LIA)进行anti—gp210和 anti—spl00的检测，对于不同检测方法之间的一致性使用Kappa进行再次检验。结果 使用化学发光法(CLIA)和酶联免疫吸附法(ELISA)对所有患者进行平行检测AMA—M2的总符合率为89.5%，且使用Kappa进行再次检验的结果为0.735，P＜0.05，数据差异明显，存在统计学意义。此外，使用CLIA和线性免疫印迹法(LIA)进行anti—gp210检测的总符合率为97.2%，使用Kappa进行再次检验的结果为0.843，P＜0.05；使用CLIA和线性免疫印迹法(LIA)进行 anti—spl00的检测，总符合率为98.5%，使用Kappa进行再次检验的结果为0.973，P＜0.05。结论 使用CLIA和ELISA进行AMA—M2的检测，具有较好的检测效果，符合率良好。使用CLIA和LIA对anti—gp210和anti—spl00进行检测，有较好的检测效果，符合率良好。
关键词： 原发性胆汁性胆管炎；特异性自身抗体；免疫检测
Abstract
Objective: To analyze the specific performance of specific autoantibodies for patients with primary biliary cholangitis using different immunoassay methods. Methods: A total of 76 patients who were hospitalized for primary biliary cholangitis during the period from January 2018 to January 2020 were selected as the main research object of this experiment. For specific autoantibodies using different immunization methods Detection. According to random grouping, 76 patients with primary biliary cholangitis served as the observation group, 50 patients with liver disease and 40 healthy patients as the control group. The chemiluminescence method (CLIA) and enzyme-linked immunosorbent assay (ELISA) were used for parallel detection of AMA-M2 in all patients, and the chemiluminescence method (CLIA) and linear immunoblotting (LIA) were used for anti-gp210 and anti-spl00. For testing, the consistency between different testing methods is checked again using Kappa. Results: The total coincidence rate of AMA-M2 was 89.5% by parallel detection of all patients using chemiluminescence method (CLIA) and enzyme-linked immunosorbent assay (ELISA), and the result of re-testing with Kappa was 0.735, P <0.05. The difference in data is obvious and statistically significant. In addition, the total coincidence rate of anti-gp210 detection using CLIA and linear immunoblotting (LIA) was 97.2%, and the results of retesting using Kappa were 0.843, P <0.05; using CLIA and linear immunoblotting (LIA) The detection of anti-spl00, the total coincidence rate was 98.5%, the results of the re-test using Kappa was 0.973, P <0.05. Conclusion : The use of CLIA and ELISA for AMA-M2 detection has a good detection effect and a good coincidence rate. Using CLIA and LIA to detect anti-gp210 and anti-spl00, it has good detection effect and good coincidence rate.
Key words： Primary Biliary Cholangitis; Specific Autoantibodies; Immunoassay
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